Chloroform fumigation and the release of soil nitrogen:  A rapid direct extraction method to measure microbial biomass Nitrogen in soil

P. C. Brookes, Andrea Landman,* G. Pruden* and D. S. Jenkinson

Soils and Plant Nutrition Department, Rothamsted Experimental Station, Harpenden,

Her&. AL5 214, U.K.

he fumigation-extraction method has certain advantages over biomass N measurements by fumigation-incubation. The problems associated with the incubation stage-the need for complete fumigant removal and for a prolonged incubation under carefully controlled conditions do not arise.